نتایج جستجو برای: c rna
تعداد نتایج: 1269407 فیلتر نتایج به سال:
مقدمه: ویروس هپاتیت c (hcv) به عنوان یکی از عوامل اصلی بیماری زا برای بیماری های مزمن کبدی محسوب می شود. مطالعات قبلی نشان داده اند که پروتئین های ویروس hcv می توانند با شبکه های تنظیمی ژن از قبیل micrornas میانکنش برقرار کنند. هدف از این مقاله بررسی اثر پروتئین core ویروس hcv بر روی بیان mir-150 در یک مدل کشت سلولی بود. مواد و روش ها: پلاسمیدهای بیان کننده طول کامل پروتئین core ویروس hcv به د...
background : gb virus c (gbv-c) is a flavivirus that was characterized in 1995. the prevalence of gbv-c rna in hiv-infected subjects has not been previously studied in iran and was therefore determined. methods: we have investigated serum samples of 80 patients from imam khomeini hospital, tehran, iran, whose hiv infection was confirmed in our laboratory by western-blotting. we used nested-pc...
background hepatitis c virus (hcv) infection has been explained as a disease that sometimes present with rheumatic manifestations indistinguishable from rheumatoid arthritis. this study has been performed to evaluate the frequency of hepatitis c virus infection in a group of patients with rheumatoid arthritis. patients and methods in this study, during one year, serum samples collected from two...
results phylogenetic analysis of the gene revealed that pakistani 1a hcv strains are in the start of third cluster and there is a difference between inter pakistani isolates at primary, secondary and tertiary levels. material and methods patient sera infected with hepatitis c virus, genotype 1a, were obtained from molecular diagnostics lab, cemb, university of the punjab lahore by using bd vacu...
Background: Groundnut bud necrosis virus (GBNV) (Tospovirus genus, Bunyaviridae family) infects the major crops of solanaceae, leguminosae and cucurbitaceae in India. Temperature is an important factor which influences the plant growth and development under diseased conditions.Objective: In the present study, we evaluated the effect of four different temp...
background the rate of mother-to-infant hepatitis c virus (hcv) transmission is relatively low, however, this mode of infection is the most important route for childhood hcv involvement. the chances of transmission are increased by the level of maternal hcv viraemia and maternal human immunodeficiency virus (hiv) co infection. objectives the objective of the present study was to determine the p...
مولکول rna دنباله ای از چهار نوکلئوتید g، c، a و u است که نقش مهمی را در فرآیندهای زیستی انجام می دهد. این نوکلئوتیدها به کمک پیوندهای هیدروژنی به یکدیگر متصل شده و در نتیجه هر مولکول rna در داخل سلول به شکل خاص (ساختار) درآمده و شکل این ساختار، عملکرد مولکول rna را مشخص می کند. پیش بینی ساختار rna یکی از مسائل مهم در علم بیوانفورماتیک می باشد که توجه ویژه ای در سال های اخیر به آن شده است. به د...
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extracti...
background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...
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